Composite

Part:BBa_M36553:Design

Designed by: Aprotim Cory Bhowmik, Cali Nguyen, Zoe Lee-Chiong   Group: Stanford BIOE44 - S11   (2015-10-22)


OYE6 Composite Sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 1199
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 1199
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 1199
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 1199
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 91
    Illegal EcoRI site found at 1199
    Illegal NgoMIV site found at 314
    Illegal NgoMIV site found at 547
    Illegal NgoMIV site found at 1165
    Illegal AgeI site found at 140
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This design was done using a DNA2.0 software and GeneDesigner2.0 quality checks. The recommended design (in terms of promoters, RBS, tags, and terminators) was taken without amendments.


Source

All of these are basic parts that have each of their sources documented individually.

References